GSE136102 Profile of new early pregnancy markers identified by transcriptomic analysis in peripheral blood immune cells in beef heifers

Contributors : Guilherme Pugliesi ; S ónia C da Silva AndradeSeries Type : Expression profiling by high throughput sequencingOrganism : Bos indicusWe aimed with this study through RNAseq, found diferentially expressend genes (DEGs) that can be used as a new pregnancy markers in peripheral blood mononuclear cells (PBMC) on day 18 post artificial insemination; and to assess the mRNA profile by RT-qPCR of new pregnancy markers in PBMC and peripheral blood polymorphonuclear cells (PMN) at early pregnancyMethods: Nelore heifers (N=21) were artificially inseminated in a fixed time (D0). On Day 10, 14, 16, 18 and 20, blood was collected for isolation of PBMC and PMN, and P4 concentration assay and color Doppler ultrasonography was performed to evaluate the corpus luteum (CL) . Pregnancy diagnosis was done on Day 28 and heifers were classified in pregant (N=9) and non-pregant (N=12). Heifers (N=6/group) with different (P

Source: GEO: Gene Expression Omnibus - August 22, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Bos indicus Source Type: research