Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophila and its evaluation for vanillin synthesis

Publication date: 10 October 2019Source: Journal of Biotechnology, Volume 304Author(s): Melissa Horvat, Giuseppe Fiume, Susanne Fritsche, Margit WinklerAbstractA novel type III fungal CAR was identified from the organism Thermothelomyces thermophila. High expression levels were observed in E. coli using the pETDuet-1 plasmid system in combination with an autoinduction protocol. A broad substrate scope ranging from aromatic to aliphatic carboxylic acids was tested and TtCAR showed activity for all substrates. High specific activities for aromatic substrates and short chain aliphatic substrates were observed, comparable to those of NcCAR, the first type III fungal CAR. TtCAR’s pH and temperature optima were at 6.5 and 30 °C, respectively. Up to 20% (v/v) cosolvents did not show a decrease in specific activity of TtCAR using (E)-cinnamic acid as a substrate. Its half-life at 40 °C was determined to be 8.25 h and its melting temperature (Tm) was 56 °C. In vitro reactions with TtCAR reduced 95.2% of 10 mM vanillic acid, which correlated to a titer of 1.4 g L−1 of vanillin. The space time yield of 0.029 g L-1 h-1 indicates that further improvements would be necessary for an industrially relevant application. This would be especially important when competing against de novo synthesis of bio vanillin by microbial strains producing>30 g L-1. In de novo and in vivo biosynthesis systems, by-products are fairly common. By contrast, we we...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research

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Publication date: Available online 18 September 2019Source: European Polymer JournalAuthor(s): Katarina Nešović, Ana Janković, Tamara Radetić, Maja Vukašinović-Sekulić, Vesna Kojić, Ljiljana Živković, Aleksandra Perić-Grujić, Kyong Yop Rhee, Vesna Mišković-StankovićAbstractIn this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas...
Source: European Polymer Journal - Category: Chemistry Source Type: research
Publication date: Available online 10 September 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): K.P. Anjali, B.M. Sangeetha, Geetha Devi, R. Raghunathan, Susmita DuttaAbstractSeaweeds are considered to be one of the richest bio-reserves, comprising of numerous bioactive compounds with versatile properties and multiple activities. The present study examined the antibacterial activity of two types of seaweeds, Ulva lactuca (green) and Stoechospermum marginatum (brown) collected from Oman Coastal region against five multidrug-resistant bacteria. The aqueous extracts of the seaweeds showed better an...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method. The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria. Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity. The films showed thermal stability and higher mechanical properties as compared to pure PVA films. In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibrobla...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Abstract Recently, nanocomposite nanofibers have been extensively used for biomedical applications. It is expected that simultaneous incorporation of antibiotic drugs and ZnO nanoparticles into nanofiber resulted in providing the synergistic anti-bacterial effect. The main aim of the present study is to fabricate polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC)-ZnO nanocomposite fibrous mats containing erythromycin (EM) drug and crosslink them using 2% glutaraldehyde vapor and 3% AlCl3 alcoholic solution. The fabricated nanofibers characterized via TGA, FTIR, TEM, and SEM, indicating that the addition of ZnO ...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Publication date: Available online 7 September 2019Source: Journal of Membrane ScienceAuthor(s): Zhen Wang, Feng Yan, Hongchang Pei, Kaiyu Yan, Zhenyu Cui, Benqiao He, Kuanjun Fang, Jianxin LiAbstractAn environmentally-friendly halloysite nanotubes@chitosan/polyvinyl alcohol/non-woven fabric (HNTs@CS/PVA/NWF) hybrid membrane with a uniform hierarchical porous structure for air filtration was prepared using a developed dip coating method combined with nonsolvent induced phase separation (NIPS) and the surface deposition of HNTs. Additionally, a TiO2/H2O2 photocatalysis approach was developed to improve the hydrophilicity of...
Source: Journal of Membrane Science - Category: Materials Science Source Type: research
Non-alcoholic fatty liver disease (NAFLD) has emerged as one of the leading liver diseases worldwide. NAFLD is characterised by hepatic steatosis and may progress to an inflammatory condition termed non-alcoholic steatohepatitis, (NASH), liver cirrhosis and hepatocellular carcinoma. It became evident in the last years that NAFLD pathophysiology is complex and involves diverse immunological and metabolic pathways. An association between intestinal signals (e.g. derived from the gut microbiota) and the development of obesity and its metabolic consequences such as NAFLD are increasingly recognized. Preclinical studies have sh...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method. The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria. Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity. The films showed thermal stability and higher mechanical properties as compared to pure PVA films. In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibrobla...
Source: Colloids and Surfaces - Category: Biotechnology Authors: Tags: Colloids Surf B Biointerfaces Source Type: research
This study thus quantifies the benefits of incorporating PPE and SD into PVA films for potential application as active packaging films or coatings.
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research
Abstract Post-traumatic stress disorder(PTSD) is associated with cirrhosis in Veterans and therapeutic results are suboptimal. An altered gut-liver-brain axis exists in cirrhosis due to hepatic encephalopathy(HE), but the added impact of PTSD is unclear. Aim: Define linkages between gut microbiota and cognition in cirrhosis with/without PTSD. Cirrhotic Veterans (with/without prior HE) underwent cognitive testing (PHES, inhibitory control test,ICT and block design BDT), serum lipopolysaccharide-binding protein(LBP) and stool collection for 16SrRNA microbiota composition and predicted function analysis(PiCRUST). PTS...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - Category: Physiology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research
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Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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