Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans

Publication date: Available online 16 August 2019Source: Acta Pharmaceutica Sinica BAuthor(s): Yisong Liu, Qi Tang, Pi Cheng, Mingfei Zhu, Hui Zhang, Jiazhe Liu, Mengting Zuo, Chongyin Huang, Changqiao Wu, Zhiliang Sun, Zhaoying LiuAbstractGelsemium elegans (G. elegans) (2n=2x=16) is genus of flowering plants belonging to the Gelsemicaeae family. Here, we report a high-quality genome assembly using the Oxford Nanopore Technologies (ONT) platform and high-throughput chromosome conformation capture techniques (Hi-C). A total of 56.11 Gb of raw GridION X5 platform ONT reads (6.23 Gb per cell) were generated. After filtering, 53.45 Gb of clean reads were obtained, giving 160×coverage depth. The de novo genome assemblies 335.13 Mb, close to the 338 Mb estimated by k-mer analysis, was generated with contig N50 of 10.23 Mb. The vast majority (99.2%) of the G. elegans assembled sequence was anchored onto 8 pseudo-chromosomes. The genome completeness was then evaluated and 1338 of the 1440 conserved genes (92.9%) could be found in the assembly. Genome annotation revealed that 43.16% of the G. elegans genome is composed of repetitive elements and 23.9% is composed of long terminal repeat elements. We predicted 26,768 protein-coding genes, of which 84.56% were functionally annotated. The genomic sequences of G. elegans could be a valuable source for comparative genomic analysis in the Gelsemicaeae family and will be useful for understanding the phylogenetic relationships of the indole ...
Source: Acta Pharmaceutica Sinica B - Category: Cancer & Oncology Source Type: research