Nitrogen flow and microbial community in the anoxic reactor of “Sulfate Reduction, Denitrification/Anammox and Partial Nitrification” process

Publication date: Available online 11 August 2019Source: Biochemical Engineering JournalAuthor(s): Yuka Kosugi, Norihisa Matsuura, Qiaochu Liang, Ryoko Yamamoto-IkemotoAbstractWe propose a sulfate reduction, denitrification/anammox and partial nitrification process to treat organic wastewater. Nitrogen removal in the anoxic reactor in this process was examined using an anaerobic-anoxic biofilter reactor. When nitrate medium was added to the anoxic reactor simulating the return flow from a nitrification reactor, mainly heterotrophic and sulfur denitrification occurred. However, anammox as well as denitrification occurred in the anoxic reactor when nitrite was fed into the reactor simulating partially nitrified water. The proportion of nitrite removal by the anammox reaction reached over 30%. By 16S rRNA gene sequencing analysis, the coexistence of heterotrophic and sulfur denitrification bacteria was confirmed in the anoxic reactor, although the heterotrophic denitrification and sulfur oxidation bacterial communities were different in each reactor. Candidatus Brocadia was detected in the nitrite-fed reactor, meaning that anammox bacteria coexist with denitrification bacteria even in high-sulfide conditions.Graphical abstract
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research