Correlative FLIM-Confocal-Raman mapping applied to plant lignin composition and autofluorescence

We present a method for correlative FLIM-confocal-Raman imaging and its application to lignin composition studies in the woody stems of the plant model Arabidopsis thaliana. Lignin is autofluorescent and exhibits characteristic fluorescence lifetimes attributed to its composition. Its composition can be further resolved by Raman microscopy to multiple peaks that represent different components. A lignin biosynthetic mutant is found to have a marked difference in fluorescence lifetime and corresponds to a change in composition as demonstrated by the Raman output.
Source: Micron - Category: Biology Source Type: research