Size-dependent antibacterial activity of copper nanoparticles against Xanthomonas oryzae pv. oryzae – A synthetic and mechanistic approach

Publication date: September 2019Source: Colloid and Interface Science Communications, Volume 32Author(s): Tapodhara Datta Majumdar, Mukesh Singh, Mala Thapa, Moumita Dutta, Abhishek Mukherjee, Chandan Kumar GhoshAbstractXanthomonas oryzae pv. oryzae (X. oryzae), causing devastating bacterial leaf blight (BLB) disease in rice, is fast developing resistance against many available antibiotics, warranting an urgent need to develop an alternative control strategy. In this manuscript, we report copper nanoparticles (CuNPs) as an effective antibacterial agent against X. oryzae. We synthesized four different sized CuNPs in a narrow-size range (CuNP-1 – 18 nm, CuNP-2 – 24 nm, CuNP-3 – 28 nm and CuNP-4 – 33 nm) by chemical reduction method. The physicochemical characterizations of CuNPs were done by X-ray diffraction (XRD), dynamic light scattering (DLS), UV–Visible Spectroscopy (UV–Vis Spectroscopy) and transmission electron microscopy (TEM). The antibacterial activity of the CuNPs was found to be size and concentration dependent. Among all the CuNPs, CuNP-3 exerted the best antibacterial efficiency against X. oryzae due to the increased production of reactive oxygen radicals than the others. It also displayed increased activity compared to commercial antibiotic streptomycin sulphate.Graphical abstract
Source: Colloids and Interface Science Communications - Category: Nanotechnology Source Type: research