Microwave assisted high performance liquid chromatography for the separation of triacylglycerols in vegetable oils using an evaporative light scattering detector

Publication date: Available online 17 July 2019Source: Food ChemistryAuthor(s): Silvia Carballo-Marrero, Maria Soledad Prats-Moya, Salvador E. Maestre-Pérez, José-Luis Todolí-TorróAbstractMicrowave (MW) radiation was applied to perform the separation of triacylglycerols (TGs) in oil samples. The novelty of the work lies in the application of MW radiation to assist the separation of several non-polar compounds employing a totally organic mobile phase. Once the influence of the evaporative light scattering detector (ELSD).variables on the sensitivity was optimized, the TGs separation was compared conditioning the column with either a conventional HPLC or a MW oven. Contrary to previous applications in which the mobile phase contained water, the improvement in sensitivity using MW was not as significant in comparison with conventional heating but it allowed a shortening retention times of several TGs in about 50 % respect elution at room. The method was finally applied to the quantification of most common TGs in almond, tiger nut, and argan oil.
Source: Food Chemistry - Category: Food Science Source Type: research