Purification and Characterization of a Bacteriocin, BacBS2, Produced by Bacillus velezensis BS2 Isolated from Meong-ge Jeotgal.

Purification and Characterization of a Bacteriocin, BacBS2, Produced by Bacillus velezensis BS2 Isolated from Meong-ge Jeotgal. J Microbiol Biotechnol. 2019 Jun 18;: Authors: Perumal V, Yao Z, Kim JA, Kim HJ, Kim JH Abstract Bacillus velezensis BS2 was isolated from Meong-ge (common sea squirt) jeotgal, a Korean fermented seafood, and produces a bacteriocin, BacBS2, which strongly inhibits Listeria monocytogenes and Bacillus cereus. BacBS2 was partially purified by Q-Sepharose column chromatography after ammonium sulfate precipitation of culture supernatant, then further purified by Sephadex G-50 column chromatography. Partially purified BacBS2 was estimated to be 6.5 kDa in size by Tricine-SDS PAGE and activity detection by gel-overlay. Enzyme treatment and FT-IR spectrum of partially purified BacBS2 confirmed its proteinacious nature. BacBS2 was fully stable at pH 4-9, and half of activity retained at pH 1-3. Full activity was retained after exposure to 80°C for 15 min, but half of the activity retained upon exposure to 90°C for 15 min or 100°C for 10 min. BacBS2 inhibited L. monocytogenes by bactericidal mode of action. B. velezensis BS2 and its BacBS2 seem useful as a biopreservative for fermented foods such as jeotgals. PMID: 31216789 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research