MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation.

MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation.
Cell Mol Life Sci. 2019 Jun 18;:
Authors: Kong D, He M, Yang L, Zhou R, Yan YQ, Liang Y, Teng CB
Abstract
Skeletal myogenesis is a highly coordinated process that involves cell proliferation, differentiation and fusion controlled by a complex gene regulatory network. The microRNA gene cluster miR-17-92 has been shown to be related to this process; however, the exact role of each cluster member remains unclear. Here, we show that miR-17 and miR-20a could effectively promote the differentiation of both C2C12 myoblasts and primary bovine satellite cells. In contrast, miR-18a might play a negative role in C2C12 cell differentiation, while miR-19 and miR-92a had little influence. Transcriptome and target analyses revealed that miR-17 could act on Ccnd2, Jak1 and Rhoc genes that are critical for cell proliferation and/or fusion. Notably, the addition of miR-19 could reverse the lethal effect of miR-17 and could thus facilitate the maturation of myotubes. Furthermore, by co-injecting the lentiviral shRNAs of miR-17 and miR-19 into mouse tibialis anterior muscles, we demonstrated the wound healing abilities of the two miRNAs. Our findings indicate that in combination with miR-19, miR-17 is a potent inducer of skeletal muscle differentiation.
PMID: 31214725 [PubMed - as supplied by publisher]

Source: Cellular and Molecular Life Sciences : CMLS - June 17, 2019 Category: Cytology Authors: Kong D, He M, Yang L, Zhou R, Yan YQ, Liang Y, Teng CB Tags: Cell Mol Life Sci Source Type: research