Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus virus.

In this study, we report a novel FPV lateral flow dipstick RPA (LFD-RPA) instant detection method capable of detecting a range of different FPV strains. The LFD-RPA assay consists of specific primers, probe, and nucleic acid strip. It is capable of detecting 102 copies of target nucleic acid per reaction, which is one order of magnitude higher than the sensitivity of traditional PCR. The most suitable reaction conditions for this assay are at 38 ℃ for 15 min. This paper develops an efficient visual detection system that can eliminate the need for professional staff and expensive and sophisticated equipment for field detection.
PMID: 31216435 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - June 15, 2019 Category: Virology Authors: Wang ZH, Wang XJ, Hou SH Tags: J Virol Methods Source Type: research