Visualizing the Balbiani Body in Zebrafish Oocytes.

Visualizing the Balbiani Body in Zebrafish Oocytes.
Methods Mol Biol. 2019;1920:277-293
Authors: Lee KL, Marlow FL
Abstract
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
PMID: 30737697 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 20, 2019 Category: Molecular Biology Authors: Lee KL, Marlow FL Tags: Methods Mol Biol Source Type: research