Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4 + ETEC infection

In this study, we demonstrate that F4+ ETEC induce IL-17C mRNA and protein expression in intestinal tissues as well as in porcine intestinal epithelial cells (IPEC-J2). This IL-17C production is largely dependent on TLR5 signaling in IPEC-J2 cells. Both F4+ ETEC infection and exogenous IL-17C increased the expression of antimicrobial peptides and tight junction proteins, such as porcine beta-defensin (pBD)-2, claudin-1, claudin-2 and occludin in IPEC-J2 cells. Taken together, our data demonstrate that TLR5-mediated IL-17C expression in intestinal epithelial cells enhances mucosal host defense responses in a unique autocrine/paracrine manner in the intestinal epithelium against ETEC infection.
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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In this study, nine different types of deletion strains were constructed from the aforementioned four genes to explore their effects on cadaverine production in a T7 promoter based systemic bicistronic cadA-cadB transformed recombinant E. coli. Results show that all the deletion strains could increase cadaverine productivity compared to the deletion-free strain when the culture was supplemented with L-lysine. The cadaverine production from L-lysine with the deletion strains increased 3 folds when compared with that of the deletion-free strain at the cultivation time of 16 h. This indicates that the deletion strain c...
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research
In this study, a computational method is proposed to calculate irradiance and fluence rate based on the LED’s relative radiation profile and its radiant power, which are two standard pieces of information available in an LED’s datasheet. UV-LED was modeled as a polychromatic point source with a specific radiation profile and the fluence rate was calculated by solving the Radiative Transfer Equation by considering the refraction, reflection, and absorption of the medium. The irradiance and fluence rate predictions on the surface of the water were evaluated successfully by radiometry and actinometry measurements,...
Source: Journal of Photochemistry and Photobiology A: Chemistry - Category: Chemistry Source Type: research
Publication date: February 2020Source: The Lancet Global Health, Volume 8, Issue 2Author(s): Kirkby D Tickell, Rumana Sharmin, Emily L Deichsel, Laura M Lamberti, Judd L Walson, A S G Faruque, Patricia B Pavlinac, Karen L Kotloff, Mohammod J ChistiSummaryBackgroundHost vulnerabilities associated with acute malnutrition could facilitate the ability of specific enteric pathogens to cause diarrhoea and associated mortality. Using data from the Global Enteric Multicenter Study, we assessed whether acute malnutrition modifies the association between common enteric pathogens and moderate-to-severe diarrhoea, and whether associat...
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Conclusions: The present study is the largest to show the high incidence and characteristics of cholangitis after KPE in patients with biliary atresia. Enterococcus is a common pathogen of cholangitis after KPE and should be considered when choosing empiric antimicrobial therapy.
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Original Articles: Hepatology Source Type: research
Publication date: July 2020Source: Journal of Food Engineering, Volume 276Author(s): Luís Marangoni Júnior, Léa Mariza de Oliveira, Paula Fernanda Janetti Bócoli, Marcelo Cristianini, Marisa Padula, Carlos Alberto Rodrigues AnjosAbstractThe effect of high-pressure processing on the morphological surface, thermal and mechanical properties of three PA and EVOH based multilayer flexible packaging materials were evaluated. LDPE/PA/LDPE, LDPE/EVOH/LDPE and PET/LDPE/PA/EVOH/PA/LDPE packaging samples were processed under three different conditions (600 MPa/25 °C/10 min, 600 MPa/90 &d...
Source: Journal of Food Engineering - Category: Food Science Source Type: research
Publication date: Available online 22 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Esther S. Taxon, Lila P. Halbers, Stanley M. ParsonsAbstractTwo groups of metabolically related enzymes, the Group III family of Fe2+-dependent alcohol dehydrogenases (ADHs) and the separate subfamily of nucleoside diphosphates linked to x (nudix) hydrolases that activate Group III ADHs are under-characterized. Here we report the steady-state initial-velocity forward direction (alcohol → aldehyde) reaction of a Group III ADH, namely gamma-hydroxybutyrate dehydrogenase (GHBDH, UniProt: Q5910...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research
Publication date: Available online 22 January 2020Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Feng Xue, Charles D. Cox, Navid Bavi, Paul R. Rohde, Yoshitaka Nakayama, Boris MartinacAbstractMechanosensitive (MS) channels have an intimate relationship with membrane lipids that underlie their mechanosensitivity. Membrane lipids may influence channel activity by directly interacting with MS channels or by influencing the global properties of the membrane such as elastic area expansion modulus or bending rigidity. Previous work has implicated membrane stiffness as a potential determinant of the mechanos...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
In this study, we aimed at developing an efficient biocatalytic process for bio-production of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP). First, adenylate cyclase from Escherichia coli MG1655 (EAC) and Bordetella Pertussis (BAC) were expressed in E. coli BL21(DE3) and comparatively analyzed for their activities. As a result, EAC from E. coli MG1655 exhibited a higher activity. However, amount of EAC were obtained in an insoluble form. Therefore, we expressed the first 446 amino acids of EAC (EAC446) to avoid the inclusion body. The effects of induction temperature, incubation time, and incubati...
Source: Chinese Journal of Chemical Engineering - Category: Chemistry Source Type: research
Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Ahmed gamal El-ShamyAbstractIn this literature, magnesium peroxide (MgO2) nanorods with an average Ddiameter = 166 nm have been successfully synthesized via fast and simple microwave assisted hydrothermal reaction for photocatalysis and antibacterial applications, for the first time. The as synthesized MgO2 nanorods are characterized by various techniques like XRD, FTIR, TEM, FE-SEM and BET. The photocatalytic activity of MgO2 nanorods has been studied for methyl orange (MO) degradation, and it is found that MO dye ha...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Inorganic ChemistryDOI: 10.1021/acs.inorgchem.9b03298
Source: Inorganic Chemistry - Category: Chemistry Authors: Source Type: research
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