ASAP Morphological Deconvolution of Beta-Lactam Polyspecificity in E. coli

ACS Chemical BiologyDOI: 10.1021/acschembio.9b00141
Source: ACS Chemical Biology - Category: Chemistry Authors: Source Type: research

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Abstract Extracellular electron transfer from the biofilm surface to the electrode is the key step for the microbial fuel cell (MFC). More recently, graphene has attracted tremendous attentions for bioelectrochemical applications due to its good biocompatibility, high electrical conductivity and large surface area. In the current work, we report a facile and green synthesis of graphene-modified carbon paper (CP) as an efficient MFC anode through plant-mediated bioreduction coupled with self-assembly. Three-dimensional CFP uniformly wrapped by curled and wrinkled biosynthesized graphene enables more surface area fo...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Publication date: Available online 15 August 2019Source: Innovative Food Science &Emerging TechnologiesAuthor(s): Hiba N. Rajha, Anna-Maria Abi Khattar, Sally El Kantar, Nadia Boussetta, Nikolai Lebovka, Richard G. Maroun, Nicolas Louka, Eugene VorobievAbstractThe effects of aqueous extraction of bioactive compounds from pomegranate peels using conventional extraction (CE) and extraction assisted by infrared irradiation (IR), ultrasound (US), pulsed electric fields (PEF), and high-voltage electrical discharges (HVED) have been compared. For the extractions assisted by US, PEF and HVED, the saturation in extraction was ...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
ta AC Abstract Human enteroviruses (EVs) are associated with a wide spectrum of human diseases. Here we report the complete genome sequences of one EV-C99 strain and one E29 strain obtained from children suffering from acute gastroenteritis, without symptoms of enteroviral syndromes. This is the first report of EV-C99 in South America, and the second E29 genome described worldwide. Continuous surveillance on EVs is vital to provide further understanding of the circulation of new or rare EV serotypes in the country. The present study also highlights the capacity of EVs to remain in silent circulation in populations...
Source: Memorias do Instituto Oswaldo Cruz - Category: Infectious Diseases Authors: Tags: Mem Inst Oswaldo Cruz Source Type: research
Authors: Moustakas I, Dryllis G, Pouliakis A, Petrikos G, Daikos G, Pittaras T, Karasante P, Karampotsis K, Tsiodras S Abstract Bacterial urogenital infections caused by multi-drug resistant organisms (MDROs), are increasingly becoming a severe public health issue. The purpose of the present study was to examine the epidemiology of recurrent UTIs along with antimicrobial resistance patterns in a cohort of patients followed as outpatients at an Infectious Disease clinic of a tertiary care center in Greece. One hundred, sequential patients suffering from recurrent UTIs and coming for clinical evaluation, follow-up an...
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
AbstractRecent studies show that rectal colonization with low-level ciprofloxacin-resistantEscherichia coli (ciprofloxacin minimal inhibitory concentration (MIC) above the epidemiological cutoff point, but below the clinical breakpoint for resistance), i.e., in the range>  0.06–0.5 mg/L is an independent risk factor for febrile urinary tract infection after transrectal ultrasound-guided biopsy (TRUS-B) of the prostate, adding to the other risk posed by established ciprofloxacin resistance inE. coli (MIC  >  0.5 mg/L) as currently defined. We aimed to identify the quinolone that by...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
We present our study on the efficacy and phenotypic impact of compensatory evolution inEscherichia coli strains carrying multiple resistance mutations. We have demonstrated that drug-resistance frequently declines within 480 generations during exposure to an antibiotic-free environment. The extent of resistance loss was found to be generally antibiotic-specific, driven by mutations that reduce both resistance level and fitness costs of antibiotic-resistance mutations. We conclude that phenotypic reversion to the antibiotic-sensitive state can be mediated by the acquisition of additional mutations, while maintaining the ori...
Source: eLife - Category: Biomedical Science Tags: Evolutionary Biology Source Type: research
Antimicrobial resistance among the bacteria present in ready-to-eat foods like vegetable salads is an emerging concern today. The current study was undertaken to investigate the presence of multi-drug resistan...
Source: BMC Research Notes - Category: Research Authors: Tags: Research note Source Type: research
Publication date: Available online 14 August 2019Source: Protein Expression and PurificationAuthor(s): Zhe Wang, Chenhao Xin, Caiming Li, Zhengbiao Gu, Li Cheng, Yan Hong, Xiaofeng Ban, Zhaofeng LiAbstractA gene encoding 1,4-α-glucan branching enzyme (GBE, EC 2.4.1.18) from the extremely thermophilic bacterium Rhodothermus obamensis STB05 was successfully cloned and expressed in Escherichia coli. Extracellular expression of the recombinant enzyme (R.o-GBE) was achieved with a yield of 1080 mg/L. Then it was purified and further characterized biochemically. R.o-GBE was optimally active at pH 7.0 and 65 °C. It ...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
In this study, we cloned and recombinantly produced five novel ADHs from Lactobacillus reuteri DSM20016 in Escherichia coli BL21 (DE3). The resulting proteins have molecular weights between 35 and 39 kDa. The optimum pH for enzyme activity of ADH-1, ADH-2 and ADH-3 was weakly acidic (pH 5, 6 and 5, respectively), compared with pH 7 for ADH-4 and ADH-5. The optimum temperature range for ADH-1, ADH-3, ADH-4 and ADH-5 was 40 °C ―55 °C, compared with 35 °C for ADH-2. These enzymes reduced a broad range of substrates; the highest catalytic efficiency and substrate affinity for ADH-1 was...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 14 August 2019Source: Journal of Organometallic ChemistryAuthor(s): Yuting Liu, Lisha Yang, Dawei Yin, Yang Dang, Lan Yang, Qian Zou, Jie Li, Jiaxi SunAbstractA novel efficient method for the synthesis of ferrocenyl chalcone Schiff bases and their metal (II) complexes has been developed. Schiff bases were synthesized with TsOH (p-toluenesulfonic acid) as catalyst and complexed with metal (II) salts (Zn (II), Pb (II), Cd (II), Ni (II)). The compounds were characterized by various spectroscopic techniques and elemental analysis. The thermal stability of the complexes was performed by thermo...
Source: Journal of Organometallic Chemistry - Category: Chemistry Source Type: research
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