An improved protocol for ICP-MS-based assessment of the cellular uptake of metal-based nanoparticles

Publication date: Available online 5 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Joanna Kruszewska, Agnieszka Kur, Dominika Kulpińska, Ilona Grabowska-Jadach, Magdalena Matczuk, Bernhard K. Keppler, Andrei R. Timerbaev, Maciej JaroszAbstractAlong with a growing interest in biomedical applications of metal-based nanoparticles, there is a compelling need in systematic information on their behavior in human body systems, preferably at the cellular level. However, in most of the in-vitro uptake experiments, the nanomaterial was applied in its native form that in reality can hardly reach the cell. In this work, we developed an improved procedure in which prior to addition to the cells the particles are converted into the protein conjugates by incubation in human serum. The procedure was tested for gold nanoparticles of different size, chosen as a representative nanomaterial on multifunctional medicinal use, and MCF-7 cell line. Using ICP-MS to measure intracellular metal concentration, it was shown that an original state has significant effect on particle internalization. The protein corona significantly inhibits the uptake amount by MCF-7 cells, with the greatest influence (a 15-fold decrease compared to uncoated particles) being exerted over the smallest, 5-nm particles (2.9 pg Au/cell). Conjugates of larger particles (20 and 50 nm) are taken up more effectively (45.0 and 33.7 pg Au/cell, respectively). The advanced protocol makes the upta...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research