Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection of Citrus leaf blotch virus.

In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorganisms and exogenous genes. The sensitivity of the RT-LAMP method was increased 100-fold compared to that of the conventional RT-PCR. In addition, this method was fast, simple and specific; it could provide better technical support for field diagnosis, customs quarantine and the control measures of CLBV. To our knowledge, this is the first report detecting CLBV using RT-LAMP.
PMID: 31132370 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - May 23, 2019 Category: Virology Authors: Liu H, Wu W, Tan J, Li Y, Mi W, Jiang L, Wu Y Tags: J Virol Methods Source Type: research