Isolating Rat Intestinal Explants for In Vitro Cultures.

Isolating Rat Intestinal Explants for In Vitro Cultures.
Curr Protoc Toxicol. 2019 May 23;:e79
Authors: Kothari A, Rajagopalan P
Abstract
The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them in vitro for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc.
PMID: 31120607 [PubMed - as supplied by publisher]

Source: Current Protocols in Toxicology - May 25, 2019 Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research