The detection of house dust mite Dermatophagoides farinae, Der f 2 and Zen-1 allergen-specific immunoglobulin E antibodies in dogs with atopic dermatitis in Malaysia

Publication date: Available online 22 May 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Wei Yee Chan, Gayathri Thevi Selvarajah, Mohd Mokrish Md. Ajat, Rieko Suzuki, Toshihiro TsukuiAbstractCanine atopic dermatitis (AD) is a chronic, inflammatory and pruritic allergic skin disease in dogs. House dust mites such as Dermatophagoides farinae are one of the known causative agents for the induction of canine AD worldwide. D. farinae protein Der f 2 is known as an important allergen involved in canine AD and recently, Zen-1 has also been identified as an allergenic protein. There is limited information on the prevalence and role of allergen sensitization to crude D. farinae extract (CDF), Der f 2 and Zen-1 among dogs diagnosed with AD in Malaysia. The aim of this study was to determine the proportion of CDF–, Der f 2– and Zen-1–specific reactive sera among dogs diagnosed with AD in Malaysia using an enzyme-linked immunosorbent assay (ELISA). Serum samples were collected from dogs diagnosed with AD from several veterinary clinics in Malaysia. The canine case records were retrieved and information on signalment, dermatological and non-dermatological histories, clinical presentation, food allergies, and exclusion of ectoparasitic, microbial and fungal skin infections were obtained through a survey form. All serum samples were evaluated to quantify the CDF–, Der f 2– and Zen-1–specific immunoglobulin E (IgE) levels. A total of 24.6%, 48.4% and 29.8% of dog...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research