Characterization of soluble CD39 (SolCD39/NTPDase1) from Piggy-Bac nonviral system as a tool to control the nucleotides level.

Characterization of soluble CD39 (SolCD39/NTPDase1) from Piggy-Bac nonviral system as a tool to control the nucleotides level.
Biochem J. 2019 May 13;:
Authors: Beckenkamp LR, Iser IC, Onzi GR, Fontoura DM, Bertoni APS, Sévigny J, Lenz G, Wink MR
Abstract
Extracellular ATP (eATP) and its metabolites have emerged as key modulators of different diseases and comprise a complex pathway called purinergic signaling. An increased number of tools have been developed to study the role of nucleotides and nucleosides in cell proliferation and migration, influence on the immune system and tumor progression. These tools include receptors agonists/antagonists, engineered ectonucleotidases, interference RNAs and ectonucleotidases inhibitors that allow the control and quantification of nucleotide levels. NTPDase1 (also called apyrase, ecto-ATPase, CD39)is one of the main enzymes responsible for the hydrolysis of eATP, and purified enzymes, such as apyrase purified from potato, or engineered as soluble CD39 (SolCD39), have been widely used in in vitro and in vivo experiments. However, the commercial apyrase had its effects recently questioned and SolCD39 exhibit limitations, such as short half-life and need of high doses to reach the expected enzymatic activity. Therefore, this study investigated a nonviral method to improve overexpression of SolCD39 and evaluated its impact over other enzymes of the purinergic system. Our data demonstrated that Pig...

Source: The Biochemical Journal - May 12, 2019 Category: Biochemistry Authors: Beckenkamp LR, Iser IC, Onzi GR, Fontoura DM, Bertoni APS, Sévigny J, Lenz G, Wink MR Tags: Biochem J Source Type: research