The impact of crosslinking and non-crosslinking fixatives on antigen retrieval and immunohistochemistry.

We report results from systematic studies on distinct pre-analytical conditions in IHC, immunofluorescence and electron microscopy. Parameters investigated are the impact of crosslinking and non-crosslinking fixatives (comparing formalin and PAXgene Tissue fixation) on whole tissue, subcellular structures and organelles, as well as on ultrastructure. The results generated show that minor changes in antigen retrieval conditions may have a major impact on IHC results and that protocols optimized for crosslinking fixatives may not be used for other fixatives without re-validation. Key antigen retrieval parameters such as buffers with different pH and duration of microwave treatment must be tested systematically for each antibody and fixation protocol.
PMID: 31082574 [PubMed - as supplied by publisher]

Source: New Biotechnology - May 9, 2019 Category: Biotechnology Authors: Stumptner C, Pabst D, Loibner M, Viertler C, Zatloukal K Tags: N Biotechnol Source Type: research