UFL1 modulates NLRP3 inflammasome activation and protects against pyroptosis in LPS-stimulated bovine mammary epithelial cells

In this study, we observed an elevating NLRP3, Caspase-1 activation and IL-1β secretion in mammary tissue of cows with mastitis and LPS-stimulated BMECs, and the experimental results here demonstrated that UFL1 depletion aggravated the LPS-induced NLRP3, Caspase-1 and IL-1β expression. Overexpression of UFL1 significantly suppressed the expression of NLRP3, Caspase-1 and IL-1β in BMECs. In addition, the suppression of NLRP3 inflammasome activation by UFL1 was partly mediated through the regulation of NF-κB signaling and ROS production. Furthermore, UFL1 overexpression could alleviate NLRP3 inflammasome activation-mediated pyroptosis in LPS-stimulated BMECs. These findings indicate that UFL1 can moudulate NLRP3 inflammasome activation and serve as effective strategies to diminish cell damage in inflammatory response by targeting NLRP3 inflammasome activation.
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research