Development of Molecular Marker through Genome Realignment for Specific Detection of Xanthomonas campestris pv. campestris race 5, the Pathogen of Black Rot Disease.

In this study, we developed a molecular marker specific to Xcc race 5. To do this, the available whole-genome sequence of Xcc races/strains and Xc subspecies were aligned and identified a highly variable genomic region (XccR5-89.2). Subsequently, a primer set covering the 'XccR5-89.2' region was designed and tested against the genomic DNA of Xcc races/strains, Xc subspecies and other plant infecting bacterial strains (Pseudomonas syringae pv. maculicola and Erwinia carotovora subsp. carotovora). The results showed that the 'XccR5-89.2' primer pair amplified 2172-bp fragment specific to Xcc race 5. Besides, they also amplified 1515-bp fragment for Xcc race 1 and over 3000-bp fragment for Xcc race 3. However, they did not amplify from remaining Xcc races/strains, subspecies and other bacterial strains. The 'XccR5-89.2' primer pair was further PCR amplified from race-unknown Xcc strains and ICMP8 was identified as race 5 among nine race-unknown Xcc strains. Further cloning and sequencing of the bands amplified from race 5 and ICMP8 with 'XccR5-89.2' primers revealed both carrying identical sequences. The results revealed that the 'XccR5-89.2' marker can effectively and proficiently detect, and identify Xcc race 5 from Xcc races/strains, subspecies and other plant infecting bacteria. To our knowledge, this is the first report for Xcc race 5 specific molecular marker. PMID: 31030456 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research