Preferential Secretion of Collagen Type 3 versus Type 1 from Adventitial Fibroblasts Stimulated by TGF-β/Smad3-Treated Medial Smooth Muscle Cells.

Preferential Secretion of Collagen Type 3 versus Type 1 from Adventitial Fibroblasts Stimulated by TGF-β/Smad3-Treated Medial Smooth Muscle Cells. Cell Signal. 2012 Dec 29; Authors: Goel SA, Guo LW, Shi XD, Kundi R, Sovinski G, Seedial S, Liu B, Kent KC Abstract Restenosis, or arterial lumen re-narrowing, occurs in 30-50 % of the patients undergoing angioplasty. Adaptive remodeling is compensatory enlargement of the vessel size, and has been reported to prevent the deleterious effects of restenosis. Our previous studies have shown that elevated transforming growth factor (TGF-β) and its signaling protein Smad3 in the media layer induce adaptive remodeling of angioplastied rat carotid artery accompanying an increase of total collagen in the adventitia. In order to gain insights into a possible role of collagen in Smad3-induced adaptive remodeling, here we have investigated a mechanism of cell-cell communication between medial smooth muscle cells (SMCs) and adventitial fibroblasts in regulating the secretion of two major collagen subtypes. We have identified a preferential collagen-3 versus collagen-1 secretion by adventitial fibroblasts following stimulation by the conditioned medium from the TGF-β1-treated/Smad3-expressing medial smooth muscle cells (SMCs), which contained higher levels of CTGF and IGF2 as compared to control medium. Treating the TGF-β/Smad3-stimulated SMCs with an siRNA to either CTGF or IGF2 reversed the effect of condit...
Source: Cellular Signalling - Category: Cytology Authors: Tags: Cell Signal Source Type: research