Recent Advancements in Structural Improvements of Lateral Flow Assays towards Point-of-Care Testing

Publication date: Available online 17 April 2019Source: TrAC Trends in Analytical ChemistryAuthor(s): Tohid Mahmoudi, Miguel de la Guardia, Behnaz Shirdel, Ahad Mokhtarzadeh, Behzad BaradarnAbstractLateral flow assays (LFAs) provide some of the most attractive point-of-care instruments for broad applications with simple, rapid, user-friendly, and cost-effective characteristics. However, these technologies suffer from low sensitivity, poor limit of detection, and just qualitative or semi-quantitative results that restrict their practical applications. Extensive research has been reported in this area involving sensitivity enhancement, multiplex analysis, implementation for broad analytes, and development of novel electronic readers for quantitative analysis. This review presents recently reported works for the structural improvements of LFAs considering antibody immobilization, manipulation of the membrane, structural variants of LFAs, and single and dual detection mode LFAs, all these aspects focused on the visual, fluorescent, magnetic, surface-enhanced Raman scattering, electrochemical, and nanozyme-based detection.Graphical abstractSchematics of structural improvements of lateral flow assays
Source: TrAC Trends in Analytical Chemistry - Category: Chemistry Source Type: research