RAS responsive element binding protein 1 blocks the granulocytic differentiation of myeloid leukemia cells.

RAS responsive element binding protein 1 blocks the granulocytic differentiation of myeloid leukemia cells. Oncol Res. 2019 Apr 08;: Authors: Yao J, Zhong L, Zhong P, Liu D, Yuan Z, Liu J, Yao S, Zhao Y, Chen M, Li L, Liu L, Liu B Abstract RAS responsive element binding protein 1 (RREB1) is a transcription factor which implicated in RAS signaling and multiple tumors. However, the role of RREB1 in acute myeloid leukemia has not been studied. We found that RREB1 is overexpressed in AML patients and myeloid leukemia cell lines (NB4 and HL-60), and RREB1 expression was significantly decreased during granulocytic differentiation of myeloid leukemia cells induced by all-trans retinoic acid (ATRA). Then we performed a RREB1 knockdown assay in NB4 and HL-60 cells, the results showed that knockdown of RREB1 up-regulated expression of CD11b, CEBPβ, microRNA-145 (miR-145), which hinted that knockdown of RREB1 enhanced granulocytic differentiation of myeloid leukemia cells. In addition, inhibitor of miR-145 can offset the enhanced effect on granulocytic differentiation mediated by down-regulation of RREB1. These collectively findings demonstrated that RREB1 blocks granulocytic differentiation of myeloid leukemia cells by inhibiting the expression of miR-145 and downstream targets of RAS signal pathway. These may provide a promising therapeutic target for AML patients. PMID: 30982491 [PubMed - as supplied by publisher]
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research