iTRAQ-based quantitative proteomic analysis of mycelium in different predation periods in nematode trapping fungus Duddingtonia flagrans

Publication date: Available online 13 April 2019Source: Biological ControlAuthor(s): Meng Liang, Shan Du, Wenjun Dong, Jiangtao Fu, Zehao Li, Yidan Qiao, Xiangji Yin, Fugui Nie, Xiaoye Yang, Rui WangAbstractDuddingtonia flagrans, as a representative species of nematode-trapping fungi, has a strong environmental tolerance and nematode-trapping ability and is thought of as the most promising candidate for use as a biological agent. To clarify the proteomic differences during different predation periods and to explore the molecular background of the nematode-trapping mechanism, we carried out an iTRAQ (isobaric tags for relative and absolute quantitation)-based quantitative proteomic analysis for Duddingtonia flagrans during three predatory stages (0 h, 12 h, and 48 h). A total of 4244 proteins were detected; 474 of these showed differential protein abundance from 0 h to 12 h, while 119 showed differential protein abundance from 12 h to 48 h. The 474 proteins from 0 h to 12 h were primarily associated with catalytic activity, molecular binding function, transport activity, biological regulation, stress response, biolocation and carbon utilization, while the 119 proteins from 12 h to 48 h were involved in substance and energy metabolism. Further bioinformatics analysis showed that these proteins could be mapped to various pathways, eight of which were significantly enriched after mapping to KEGG pathways from 0 h to 12 h, and substance and energy metabolism were enriched from 12 ...
Source: Biological Control - Category: Biology Source Type: research