Reconfigurable multiplexed point of Care System for monitoring type 1 diabetes patients

Publication date: Available online 13 April 2019Source: Biosensors and BioelectronicsAuthor(s): Augusto Márquez, Joan Aymerich, Michele Dei, Rosalía Rodríguez-Rodríguez, Manuel Vázquez-Carrera, Javier Pizarro-Delgado, Pablo Giménez-Gómez, Ángel Merlos, Lluís Terés, Francesc Serra-Graells, Cecilia Jiménez-Jorquera, Carlos Domínguez, Xavier Muñoz-BerbelAbstractAt the point of care (POC), on-side clinical testing allows fast biomarkers determination even in resource-limited environments. Current POC systems rely on tests selective to a single analyte or complex multiplexed systems with important portability and performance limitations. Hence, there is a need for handheld POC devices enabling the detection of multiple analytes with accuracy and simplicity. Here we present a reconfigurable smartphone-interfaced electrochemical Lab-on-a-Chip (LoC) with two working electrodes for dual analyte determination enabling biomarkers' selection in situ and on-demand. Biomarkers selection was achieved by the use of electrodepositable alginate hydrogels. Alginate membranes containing either glucose oxidase (GOx) or lactate oxidase (LOx) were selectively electrodeposited on the surface of each working electrode in around 4 min, completing sample measurement in less than 1 min. Glucose and lactate determination was performed simultaneously and without cross-talk in buffer, fetal bovine serum (FBS) and whole blood samples, the latter being possible by the size-exclusion filtrati...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research