Permselective glucose sensing with GLUT1-Rich cancer cell membranes

Publication date: Available online 12 April 2019Source: Biosensors and BioelectronicsAuthor(s): Insu Kim, Dohyung Kwon, Dongtak Lee, Gyudo Lee, Dae Sung YoonAbstractEnzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from an MDA-MB-231 cell and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-(-)-fructose, D-(+)-xylose, D-(+)-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35–7.35 mM of glucose), implying their high potential for practical use.
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research