LncRNA DYNLRB2-2 inhibits THP-1 macrophage foam cell formation by enhancing autophagy.

LncRNA DYNLRB2-2 inhibits THP-1 macrophage foam cell formation by enhancing autophagy.
Biol Chem. 2018 Dec 01;:
Authors: Li Y, Sun T, Shen S, Wang L, Yan J
Abstract
The aim of this study was to investigate whether LncRNA DYNLRB2-2 can inhibite foam cell formation by activating autophagy. The location of DYNLRB2-2 in THP-1-derived macrophages was analyzed by fluorescence in situ hybridization. ox-LDL was used to induce the formation of foam cells, Oil Red O staining and HPLC were performed to detect accumulation of lipid dropletsand the level of cholesterol concentration, respectively. The mRNA and protein level of ABCA1 were examined by qRT-PCR and Western blotting. Relative protein levels of (p-)LKB1, (p-)AMPK, (p-)mTOR and autophagy markers (LC3 II, Beclin-1 and p62) in THP-1 macrophage-derived foam cells were analyzed by Western blotting. The levels of inflammatory factors (TNF-α, IL-6 and IL-1β) in THP-1 macrophagederived foam cells were detected by ELISA assay. 3-MA, and compound C were used to block autophagy. Our data show that DYNLRB2-2 inhibited formation of THP-1 macrophage-derived foam cell and promotes cholesterol efflux by activating autophagy. DYNLRB2-2 caused autophagy by activating the signaling pathway of LKB1/AMPK/mTOR in foam cells. DYNLRB2-2 activated the LKB1/AMPK/mTOR signaling pathway via miR- 298/SIRT3 axis. Our data indicated that DYNLRB2-2 enhanced cholesterol efflux by regulating the LKB1/AMPK/mTOR autoph...

Source: Biological Chemistry - March 25, 2019 Category: Chemistry Tags: Biol Chem Source Type: research