NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Publication date: Available online 13 March 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Sameh Obeid, Pei-Shan Sung, Benoit Le Roy, Ming-Li Chou, Shie-Lang Shieh, Celine Elie-Caille, Thierry Burnouf, Wilfrid BoireauAbstractPlasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25–1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.Graphical AbstractBlood products for transfusion are essential medicinal products. Blood collection and processing may induce platelet activation leading to generation of platelet extracellul...

Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 14, 2019 Category: Nanotechnology Source Type: research