Population genetics reveal Myotis keenii (Keen ’s myotis) and Myotis evotis (long-eared myotis) to be a single species

Canadian Journal of Zoology,Volume 97, Issue 3, Page 267-279, March 2019. Recognizing delineations of gene flow among groups of animals can be challenging but is necessary for conservation and management. Of particular importance is the identification of species boundaries. Several physical and genetic traits have been used with mixed success to distinguish Myotis keenii (Merriam, 1895) (Keen ’s myotis) and Myotis evotis (H. Allen, 1864) (long-eared myotis), but it is unclear whether species distinction is biologically warranted. We generated 12–14 microsatellite locus genotypes for 275 long-eared Myotis representing four species — M. keenii, M. evotis, Myotis septentrionalis (Trou essart, 1897) (northern myotis), and Myotis thysanodes Miller, 1897 (fringed myotis) — from across northwestern North America and 23 Myotis lucifugus (Le Conte, 1831) (little brown myotis) as the outgroup. Population genetic analyses revealed four well-defined groups (species): M. septentrionalis, M. thysanodes, M. lucifugus, and a single group comprising M. keenii and M. evotis. We document high rates of gene flow within M. evotis/M. keenii. Cytochrome b gene (mtDNA) sequencing failed to resolve morphologically identifiable species. We highlight the importance of geographically thorough inv estigation of genetic connectivity (nuclear markers) when assessing taxonomic status of closely related groups. We document a morphometric cline within M. evotis/M. keenii that may in part explain earli...
Source: Canadian Journal of Zoology - Category: Zoology Authors: Source Type: research