Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.
Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.
Methods Mol Biol. 2019;1943:365-375
Authors: Merkel OM, Marsh LM, Garn H, Kissel T
Abstract
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected has so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein (EGFP) expressing mice for the differentiation of transfected lung cell populations based on their antigen expression.
PMID: 30838629 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Merkel OM, Marsh LM, Garn H, Kissel T Tags: Methods Mol Biol Source Type: research
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