Major yolk protein and HSC70 are essential for the activation of the TLR pathway via interacting with MyD88 in Apostichopus japonicus.

Major yolk protein and HSC70 are essential for the activation of the TLR pathway via interacting with MyD88 in Apostichopus japonicus.
Arch Biochem Biophys. 2019 Mar 02;:
Authors: Lv Z, Li C, Guo M, Shao Y, Zhang W, Zhao X
Abstract
The Toll cascade plays important functions in innate immunity against infectious pathogens in animals. Toll cascade as an ancient immune defender were conserved among different species. The activation of the TLR pathway between different species often involves different interacting proteins. The core members of this pathway have been well established in a wide range of organisms, including the marine invertebrate sea cucumber. However, these proteins do not function as single isolated entities but are engaged in a dynamic physical network with other proteins in the biomolecular context of a cell. To fill the knowledge gap in this context, two novel members of major yolk protein (MYP) and heat shock cognate protein 70 (HSC70) were identified as myeloid differentiation factor 88 (MyD88) interacting proteins by GST pull-down and mass spectrometry assays in Apostichopus japonicus. Their interactions were further confirmed by a co-immunoprecipitation analysis. Confocal microscopy analysis revealed that these three proteins were co-localized in the cytoplasm. A functional experiment indicated that each protein alone could suppress NF-κB translocated in the nucleus in cultured coelomocytes via a siRNA interferen...

Source: Archives of Biochemistry and Biophysics - March 1, 2019 Category: Biochemistry Authors: Lv Z, Li C, Guo M, Shao Y, Zhang W, Zhao X Tags: Arch Biochem Biophys Source Type: research