Biochemical evaluation of phenolic compounds and steviol glycoside from Stevia rebaudiana extracts associated with in vitro antidiabetic potential

Publication date: Available online 21 February 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Uswatun Hasanah Zaidan, Nur Izzati Mohamad Zen, Nor Aida Amran, Suhaili Shamsi, Siti Salwa Abd GaniAbstractApart from being well known to the world as herb-based sweetening additive, Stevia rebaudiana (S. rebaudiana) and its phenolic compounds are considered as a natural antidiabetic alternative to replace synthetic drugs that possess numbers of side effects. Therefore, this study was aimed to evaluate the solvent effects on the extraction of phenolic compounds and steviol glycoside identification associated with antidiabetic potential of the extracts. Total phenolic (TPC) and flavonoid (TFC) content of the extracts were quantified, while the antidiabetic activity of the extracts was determined by α-amylase and α-glucosidase inhibitory assay. As a matter of interest, TFC was found to be present at the highest concentration in ethanol extract (10.91 mg QE/g), while the presence of TPC showed no significant difference between water extract (6.65 mg GAE/g) and other organic solvents. HPLC analysis showed the abundant presence of steviol glycoside in the water extract, the principal compound suggested for treating diabetes. Furthermore, GC-MS analysis has shown the major compounds of 1-heptatriacotanol, duvatrienediol, dihydroxanthin, β-amyrin, lupenone, phytol, γ-sitosterol, agatholic acid and fatty acids were present. In relation to the antidiabetic potential...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research