GSE123159 ChIP-seq analysis of transcription factor AtrR binding across the Aspergillus fumigatus genome

Contributors : Sanjoy Paul ; Mark Stamnes ; Scott Moye-RowleySeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Aspergillus fumigatus Af293To determine the genomic binding sites for the Aspergillus fumigatus transcription factor AtrR, we utilized three different strains. One was the wild-type AfS35 which contains a wild-type copy of atrR but lacks any HA tag. The other two are both derived from AfS35 but contain a 3X HA tag replacing the stop codon of atrR. These strains are SPF89 and SPF112, respectively. SPF89 uses the wild-type atrR promoter to drive expression of the epitope-tagged allele while SPF112 uses the much strong hspA promoter to drive production of the same protein.

Source: GEO: Gene Expression Omnibus - February 11, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Aspergillus fumigatus Af293 Source Type: research