“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

Publication date: Available online 29 January 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): S.I. Goreninskii, R.O. Guliaev, K.S. Stankevich, N.V. Danilenko, E.N. Bolbasov, A.S. Golovkin, A.I. Mishanin, V.D. Filimonov, S.I. TverdokhlebovAbstractIn the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.Graphical abstract

Source: Colloids and Surfaces B: Biointerfaces - January 30, 2019 Category: Biochemistry Source Type: research