Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production.

Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production.
Biochem Genet. 2019 Jan 14;:
Authors: Zhang Y, Zhang X, Xiao S, Qi W, Xu J, Yuan Z, Wang Z
Abstract
3-Methyl-1-butanol (3MB) is a promising biofuel that can be produced from 2-ketoisocaproate via the common L-leucine biosynthesis pathway. Corynebacterium glutamicum was chosen as a host bacterium because of its strong resistance to isobutanol. In the current study, several strategies were designed to overproduce 3MB in C. glutamicum through a non-fermentation pathway. The engineered C. glutamicum mutant was obtained by silencing the pyruvate dehydrogenase gene complex (aceE) and deleting the lactic dehydrogenase gene (ldh), followed by mutagenesis with diethyl sulfate (DES) and selection with Fmoc-3-4-thiazolyl-L-alanine (FTA). The mutant could produce 659 mg/L of 3MB after 12 h of incubation. To facilitate carbon flux to 3MB biosynthesis, the engineered recombinant was also constructed without branched-chain acid aminotransferase (ilvE) activity by deleting the ilvE gene. This recombinant could produce 697 mg/L of 3MB after 12 h of incubation.
PMID: 30644007 [PubMed - as supplied by publisher]

Source: Biochemical Genetics - January 14, 2019 Category: Genetics & Stem Cells Authors: Zhang Y, Zhang X, Xiao S, Qi W, Xu J, Yuan Z, Wang Z Tags: Biochem Genet Source Type: research