Identifying Weak Signals in Inhomogeneous Neuronal Images for Large-Scale Tracing of Sparsely Distributed Neurites

AbstractTracing neurites constitutes the core of neuronal morphology reconstruction, a key step toward neuronal circuit mapping. Modern optical-imaging techniques allow observation of nearly complete mouse neuron morphologies across brain regions or even the whole brain. However, high-level automation reconstruction of neurons, i.e., the reconstruction with a few of manual edits requires discrimination of weak foreground points from the inhomogeneous background. We constructed an identification model, where empirical observations made from neuronal images were summarized into rules for designing feature vectors that to classify foreground and background, and a support vector machine (SVM) was used to learn these feature vectors. We embedded this constructed SVM classifier into a previously developed tool, SparseTracer, to obtain SparseTracer-Learned Feature Vector (ST-LFV). ST-LFV can trace sparsely distributed neurites with weak signals (contrast-to-noise ratio  <  1.5) against an inhomogeneous background in datasets imaged by widely used light-microscopy techniques like confocal microscopy and two-photon microscopy. Moreover, 12 sub-blocks were extracted from different brain regions. The average recall and precision rates were 99% and 97%, respectively. These results indicated that ST-LFV is well suited for weak signal identification with varying image characteristics. We also applied ST-LFV to trace long-range neurites from images where neurites are sparsely distrib...

Source: Neuroinformatics - January 11, 2019 Category: Neuroscience Source Type: research