Chemical and topographical single-cell imaging by near-field desorption mass spectrometry.

Chemical and topographical single-cell imaging by near-field desorption mass spectrometry. Angew Chem Int Ed Engl. 2019 Jan 02;: Authors: Hang W, Yin Z, Cheng X, Liu R, Li X, Hang L, Xu J, Yan X, Li J, Tian Z Abstract Simultaneous acquiring chemical and topographical information within a single cell at nanoscale resolutions is vital to cellular biology, yet it remains a great challenge for current techniques due to limited lateral resolutions and detection sensitivities. Here we report the development of near-field desorption mass spectrometry for correlated chemical and topographical imaging, thereby bridging the gap between laser-based MS methods and multimodal single-cell imaging. Using this integrated platform, imaging resolution of 250 nm and 3D topographically reconstructed chemical single-cell imaging were achieved. This technique offers more in-depth cellular information than is currently possible with micrometre-range laser-based MS imaging methods. Considering the simplicity and compact size of the near-field device, this technique can be introduced to matrix-assisted laser desorption ionization MS, expanding the multimodal abilities of MS at nanoscale resolutions across wide disciplines. PMID: 30600882 [PubMed - as supplied by publisher]
Source: Angewandte Chemie - Category: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research