Comparative analysis and properties evaluation of gelatin microspheres crosslinked with glutaraldehyde and 3-Glycidoxypropyltrimethoxysilane as drug delivery systems for the antibiotic vancomycin

Publication date: Available online 29 December 2018Source: International Journal of PharmaceuticsAuthor(s): Mohammad Nouri-Felekori, Mehrdad Khakbiz, Nader Nezafati, Javad Mohammadi, Mohamadreza Baghban EslaminejadAbstractIn the present comparative study, gelatin microspheres (GMs) were prepared by Emulsification-solvent-extraction method using well-known crosslinker: Glutaraldehyde (GA) and biocompatible silane-coupling agent: Glycidoxypropyltrimethoxysilane (GPTMS). Crosslinking with GA was done by a definite and common procedure, while GPTMS crosslinking potency was investigated after 5, 10, 24, and 48 h synthesis periods and the fabrication method was adjusted in order for preparation of GMs with optimized morphological and compositional characteristics. The prepared GMs were then evaluated and compared as drug delivery systems for the antibiotic vancomycin (Vm). Morphological observations, FTIR, ninhydrin assay, swelling behavior evaluation and Hydrolytic degradation analysis proved successful modification of GMs and revealed that increasing synthesis time from 5 h to 24 h and 48 h, when using GPTMS as crosslinker, led to formation of morphologically-optimized GMs with highest crosslinking degree (∼ 50%) and the slowest hydrolytic degradation rate. Such GMs also exhibited most sustained release period of Vm. The antibacterial test results against gram-positive bacterium Staphylococcus aureus, were in accordance with the release profiles of Vm, as well. Together, GPTMS-...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research