Survival and growth in vitro of Paramoeba perurans populations cultured under different salinities and temperatures

Publication date: Available online 21 December 2018Source: ProtistAuthor(s): Catherine Collins, Malcolm Hall, Mark J. Fordyce, Patricia WhiteAbstractGrowth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at salinities between 25‰ and 50‰, 50‰ being the maximum salinity tested. Amoebae numbers were sustained at 4 °C. Therefore, lower temperature and salinity thresholds for P. perurans population growth lie between 4 to 8 °C, and salinities of 20 to 25‰, respectively. Upper limits were not determined in this study. The populations remained relatively stable at 4 °C and 2 °C at permissive salinities with respect to numbers of viable amoebae over the 15 day exposure period.
Source: Protist - Category: Molecular Biology Source Type: research