Phylogenetic Revision of the Order Entactinaria—Paleozoic Relict Radiolaria (Rhizaria, SAR)
Publication date: Available online 7 January 2020Source: ProtistAuthor(s): Yasuhide Nakamura, Miguel M. Sandin, Noritoshi Suzuki, Akihiro Tuji, Fabrice NotEntactinaria, an order of Radiolaria, are defined by a specific skeletal structure called “initial spicular system (ISS)”. The oldest entactinarians appeared in the Ordovician period, and the extant species are thought to have survived until today. However, the morphological observation revealed that the ISSs of entactinarian families are highly variable, and the validity of this characteristic is questionable. This is supported by the results of 18S and 28S ...
Source: Protist - January 8, 2020 Category: Molecular Biology Source Type: research

Protist Interactions and Community Structure During Early Autumn in the Kerguelen region (Southern Ocean)
This study investigated protist community composition and biotic interactions focusing on microplankton at four distinct sites around the Kerguelen Islands (Southern Ocean) after the summer phytoplankton bloom. Protist diversity in different size fractions, sampled with Niskin bottles and plankton nets, was assessed by sequencing of the V4 18S rDNA region. Combining different approaches, i.e. sequencing of different plankton size fractions, and isolation and sequencing of single cells, provided new insights into microbial interactions in protist communities. The communities displayed high variability, including short-term ...
Source: Protist - December 18, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: December 2019Source: Protist, Volume 170, Issue 6Author(s): (Source: Protist)
Source: Protist - December 8, 2019 Category: Molecular Biology Source Type: research

Molecular phylogeny of marine gregarines (Apicomplexa) from the Sea of Japan and the Northwest Pacific including the description of three novel species of Selenidium and Trollidium akkeshiense n. gen. n. sp
This study set out to bolster morphological and molecular datasets of marine gregarine apicomplexans. Gregarines were sampled from the Sea of Japan and Northwest Pacific from cirratuliform polychaetes (Acrocirridae, Cirratulidae, and Flabelligeridae), as well as sipunculids. Trophozoites (feeding stages) were gathered for identification using light microscopy, scanning electron microscopy, and transmission electron microscopy. Cells were also collected for molecular phylogenetic analysis using 18S rDNA and 28S rDNA. As a result, three new species of Selenidium, S. planusae n. sp., S. validusae n. sp., and S. pyroidea n. sp...
Source: Protist - December 8, 2019 Category: Molecular Biology Source Type: research

Zero Intercalary Plates in Parvodinium (Peridiniopsidaceae, Peridiniales) and Phylogenetics of P. elpatiewskyi, comb. nov
Publication date: Available online 22 November 2019Source: ProtistAuthor(s): Juliane Kretschmann, Anže Žerdoner Čalasan, Barbara Meyer, Marc GottschlingParvodinium elpatiewskyi, comb. nov., is a common freshwater dinophyte without intercalary plates and with various spines on hypothecal sutures. However, the taxonomy of the species has had a complex history and its systematic placement remained unclear. The conserved type of P. elpatiewskyi, comb. nov., illustrated here for the first time using electron microscopy, is an environmental sample. Based on newly collected material from Berlin (Germany) we provide a morpholog...
Source: Protist - November 24, 2019 Category: Molecular Biology Source Type: research

SSU rDNA Phylogeny Indicates the Scale-lacking Trivalvulariida ord. nov. as a Sister Group to the Euglyphida (Cercozoa, Rhizaria)
Publication date: Available online 23 November 2019Source: ProtistAuthor(s): Ferry Siemensma, Kenneth DumackThe testate amoeba Leptogromia operculata was described by Valkanov in 1970 from marine waters. We re-discovered this species in brackish water along the North Sea in the Netherlands. Based on detailed comparison of morphology and SSU rDNA phylogeny we conclude that this species represents a sister clade to the Euglyphida (Imbricatea, Cercozoa). We further describe a similar species Trivalvularis immunda gen. nov., sp. nov. from freshwater in France and the Netherlands on basis of morphological data. Trivalvularis an...
Source: Protist - November 24, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: November 2019Source: Protist, Volume 170, Issue 5Author(s): (Source: Protist)
Source: Protist - November 9, 2019 Category: Molecular Biology Source Type: research

Quantitative Proteomic Map of the Trypanosomatid Strigomonas culicis: The Biological Contribution of its Endosymbiotic Bacterium
Publication date: Available online 1 November 2019Source: ProtistAuthor(s): Giselle V.F. Brunoro, Rubem F.S. Menna-Barreto, Aline S. Garcia-Gomes, Carolina Boucinha, Diogo B. Lima, Paulo C. Carvalho, André Teixeira-Ferreira, Monique R.O. Trugilho, Jonas Perales, Veit Schwämmle, Marcos Catanho, Ana Tereza R. de Vasconcelos, Maria Cristina M. Motta, Claudia M. d’Avila-Levy, Richard H. ValenteStrigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, which is highly integrated into the protist metabolism: it furnishes essenti...
Source: Protist - November 3, 2019 Category: Molecular Biology Source Type: research

Characterisation of Two Toxic Gambierdiscus spp. (Gonyaulacales, Dinophyceae) from the Great Barrier Reef (Australia): G. lewisii sp. nov. and G. holmesii sp. nov
In this study, we describe two new species of Gambierdiscus isolated from Heron Island in the Southern Great Barrier Reef, Queensland, Australia. These new species were analysed using light microscopy, scanning electron microscopy, and phylogenetic analyses of nuclear encoded ribosomal ITS, SSU as well as D1-D3 and D8-D10 of the LSU gene regions. Gambierdiscus lewisii sp. nov. (Po, 3', 0a, 7'', 6c,? s, 5''', 0p, 2'''') is distinguished by its strong reticulate-foveate ornamentation and is genetically distinct from its sister species, G. pacificus. Gambierdiscus holmesii sp. nov. (Po, 3', 0a, 7'', 6c, 6s?, 5''', 0p, 2'''') ...
Source: Protist - November 1, 2019 Category: Molecular Biology Source Type: research

The Draft Genome of the Small, Spineless Green Alga Desmodesmus costato-granulatus (Sphaeropleales, Chlorophyta)
Publication date: Available online 25 October 2019Source: ProtistAuthor(s): Sibo Wang, Linzhou Li, Yan Xu, Barbara Melkonian, Maike Lorenz, Thomas Friedl, Morten Petersen, Sunil Kumar Sahu, Michael Melkonian, Huan LiuDesmodesmus costato-granulatus (Skuja) Hegewald 2000 (Sphaeropleales, Chlorophyta) is a small, spineless green alga that is abundant in the freshwater phytoplankton of oligo- to eutrophic waters worldwide. It has a high lipid content and is considered for sustainable production of diverse compounds, including biofuels. Here, we report the draft whole-genome shotgun sequencing of D. costato-granulatus strain SA...
Source: Protist - October 26, 2019 Category: Molecular Biology Source Type: research

Novel Lineages of Oxymonad Flagellates from the Termite Porotermes adamsoni (Stolotermitidae): the Genera Oxynympha and Termitimonas
Publication date: Available online 21 October 2019Source: ProtistAuthor(s): Renate Radek, Katja Meuser, Samet Altinay, Nathan Lo, Andreas BruneThe symbiotic gut flagellates of lower termites form host-specific consortia composed of Parabasalia and Oxymonadida. The analysis of their coevolution with termites is hampered by a lack of information, particularly on the flagellates colonizing the basal host lineages. To date, there are no reports on the presence of oxymonads in termites of the family Stolotermitidae. We discovered three novel, deep-branching lineages of oxymonads in a member of this family, the damp-wood termite...
Source: Protist - October 22, 2019 Category: Molecular Biology Source Type: research

The Draft Genome of Hariotina reticulata (Sphaeropleales, Chlorophyta) Provides Insight into the Evolution of Scenedesmaceae
Publication date: Available online 19 October 2019Source: ProtistAuthor(s): Yan Xu, Linzhou Li, Hongping Liang, Barbara Melkonian, Maike Lorenz, Thomas Friedl, Morten Petersen, Huan Liu, Michael Melkonian, Sibo WangHariotina reticulata P. A. Dangeard 1889 (Sphaeropleales, Chlorophyta) is a common member of the summer phytoplankton of meso- to highly eutrophic water bodies with a worldwide distribution. Here, we report the draft whole-genome shotgun sequencing of H. reticulata strain SAG 8.81. The final assembly comprises 107,596,510 bp with over 15,219 scaffolds (>100 bp). This whole-genome project is publicly available...
Source: Protist - October 20, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: August 2019Source: Protist, Volume 170, Issue 4Author(s): (Source: Protist)
Source: Protist - October 17, 2019 Category: Molecular Biology Source Type: research

Evidence of Intra-individual SSU Polymorphisms in Dark-spored Myxomycetes (Amoebozoa)
Publication date: Available online 10 September 2019Source: ProtistAuthor(s): J.M. García-Martín, J.C. Zamora, C. LadoThe nuclear small subunit rRNA gene (SSU or 18S) is a marker frequently used in phylogenetic and barcoding studies in Amoebozoa, including Myxomycetes. Despite its common usage and the confirmed existence of divergent copies of ribosomal genes in other protists, the potential presence of intra-individual SSU variability in Myxomycetes has never been studied before. Here we investigated the pattern of nucleotide polymorphism in the 5' end fragment of SSU by cloning and sequencing a total of 238...
Source: Protist - September 12, 2019 Category: Molecular Biology Source Type: research

Taxon-rich Multigene Phylogenetic Analyses Resolve the Phylogenetic Relationship Among Deep-branching Stramenopiles
In this study, we performed multigene phylogenetic analyses of deep-branching Stramenopiles with improved taxon sampling. We sequenced transcriptomes of three deep-branching Stramenopiles: Incisomonas marina, Pseudophyllomitus vesiculosus and Platysulcus tardus. Phylogenetic analyses using 120 protein-coding genes and 56 taxa indicated that Pl. tardus is sister to all other Stramenopiles while Ps. vesiculosus is sister to MAST-4 and form a robust clade with the Labyrinthulea. The resolved phylogenetic relationships of deep-branching Stramenopiles provide insights into the ancestral traits of the Stramenopiles. (Source: Protist)
Source: Protist - September 6, 2019 Category: Molecular Biology Source Type: research

Unmasking “The Eldest Son of The Father of Protozoology”: Charles King
Publication date: August 2019Source: Protist, Volume 170, Issue 4Author(s): John R. DolanIn 1703 two articles appeared in the Transactions of the Royal Society, authored by an unnamed gentleman. The articles, with deference to Leeuwenhoeck, described recent observations made with a microscope. Clifford Dobell, in his biography of Leeuwenhoeck, remarked at length on the extraordinary quality of the illustrations and descriptions of “animalcules”. He declared the anonymous author to be the scion and master draughtsman of Leeuwenhoeck’s followers. Still today, one of the illustrations is credited with being ...
Source: Protist - September 1, 2019 Category: Molecular Biology Source Type: research

Gertia stigmatica gen. et sp. nov. (Kareniaceae, Dinophyceae), a New Marine Unarmored Dinoflagellate Possessing the Peridinin-type Chloroplast with an Eyespot
Publication date: Available online 30 August 2019Source: ProtistAuthor(s): Kazuya Takahashi, Garry Benico, Wai Mun Lum, Mitsunori IwatakiMarine unarmored dinoflagellates in the family Kareniaceae are known to possess chloroplasts of haptophyte origin, which contain fucoxanthin and its derivatives as major carotenoids, and lack peridinin. In the present study, the first species with the peridinin-type chloroplast in this family, Gertia stigmatica gen. et sp. nov., is described on the basis of ultrastructure, photosynthetic pigment composition, and molecular phylogeny inferred from nucleus- and chloroplast-encoded genes. Cel...
Source: Protist - September 1, 2019 Category: Molecular Biology Source Type: research

Molecular Subtyping of Blastocystis from Diverse Animals in the United Arab Emirates
In conclusion, this is the first report of Blastocystis infection in various animals in the UAE. Apart from ST4, no potentially zoonotic subtypes were detected. (Source: Protist)
Source: Protist - August 28, 2019 Category: Molecular Biology Source Type: research

New Section: Protist Genome Reports
Publication date: Available online 21 August 2019Source: ProtistAuthor(s): Michael Melkonian (Source: Protist)
Source: Protist - August 22, 2019 Category: Molecular Biology Source Type: research

Microscopical Studies on Ministeria vibrans Tong, 1997 (Filasterea) Highlight the Cytoskeletal Structure of the Common Ancestor of Filasterea, Metazoa and Choanoflagellata
Publication date: Available online 22 August 2019Source: ProtistAuthor(s): Alexander P. Mylnikov, Denis V. Tikhonenkov, Sergey A. Karpov, Claudia WylezichMinisteria vibrans (Filasterea) is a tiny amoeboid species described by Tong in 1997. It has been sporadically found in different habitats, and cultured strains were established. M. vibrans is well characterised by molecular phylogeny but until now was not ultrastructurally investigated in detail. Here, we provide the ultrastructure for this species based on a new strain isolated from oxygen-depleted water of the Baltic Sea. A thin vibrating flagellum could be observed bu...
Source: Protist - August 22, 2019 Category: Molecular Biology Source Type: research

Unmasking "The Eldest Son of the Father of Protozoology": Charles King
Publication date: Available online 7 August 2019Source: ProtistAuthor(s): John R. DolanIn 1703 two articles appeared in the Transactions of the Royal Society, authored by an unnamed gentleman. The articles, with deference to Leeuwenhoeck, described recent observations made with a microscope. Clifford Dobell, in his biography of Leeuwenhoeck, remarked at length on the extraordinary quality of the illustrations and descriptions of "animalcules". He declared the anonymous author to be the scion and master draughtsman of Leeuwenhoeck's followers. Still today, one of the illustrations is credited with being the first ...
Source: Protist - August 9, 2019 Category: Molecular Biology Source Type: research

A Transcriptome-based Perspective of Meiosis in Dinoflagellates
Publication date: Available online 1 August 2019Source: ProtistAuthor(s): David MorseThere is increasing interest in the possibility of sexual recombination in dinoflagellates, especially those symbiotic with coral, since recombination may be able to augment genetic diversity and reduce levels of coral bleaching. Several previous studies have addressed this in Symbiodinium by querying sequence databanks with a list of 51 genes termed a meiosis detection toolkit. Here, we have constructed an expanded list of 307 genes involved in meiosis in budding yeast. We find the genes involved in the major regulatory steps in yeast mei...
Source: Protist - August 2, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: July 2019Source: Protist, Volume 170, Issue 3Author(s): (Source: Protist)
Source: Protist - July 21, 2019 Category: Molecular Biology Source Type: research

Integrated Genomic and Transcriptomic Analysis of the Peridinin Dinoflagellate Amphidinium carterae Plastid
We present evidence that antisense transcripts are produced within the A. carterae plastid, but show that these transcripts undergo different end cleavage events from sense transcripts, and do not receive 3' poly(U) tails. The difference in processing events between sense and antisense transcripts may enable the removal of non-coding transcripts from peridinin dinoflagellate plastid transcript pools. (Source: Protist)
Source: Protist - July 6, 2019 Category: Molecular Biology Source Type: research

The Dancing Star: Reinvestigation of Artodiscus saltans (Variosea, Amoebozoa) Penard 1890
We present morphological data for A. saltans from different aquatic habitats of four European countries. We subjected three cells of one strain from Germany to molecular analyses and, interestingly, obtained six different rDNA sequences. Phylogenetic analyses of these SSU rDNA sequences revealed that A. saltans branches close to the amoebozoan Multicilia marina (Variosea, Amoebozoa). (Source: Protist)
Source: Protist - June 22, 2019 Category: Molecular Biology Source Type: research

From the Popularization of Microscopy in the Victorian Age: A Lesson for Today’s “Outreach”
Publication date: July 2019Source: Protist, Volume 170, Issue 3Author(s): John R. DolanIn the latter half of the Victorian Age (1837–1901) microscopy was introduced as popular past-time. Many books were published aimed at general audiences, both adult and juvenile, on microscopy. Here I consider 5 of these popular books of particular interest to protistologists as they included presentations of ‘infusoria’ or ‘animalcules’. I focus on the scientific backgrounds of the authors, from what we know of them, and the approaches taken to engage the reader based on their texts and illustrations. The p...
Source: Protist - June 13, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2019Source: Protist, Volume 170, Issue 2Author(s): (Source: Protist)
Source: Protist - May 28, 2019 Category: Molecular Biology Source Type: research

Metabolic Consequences of Cobalamin Scarcity in the Diatom Thalassiosira pseudonana as Revealed Through Metabolomics
Publication date: Available online 28 May 2019Source: ProtistAuthor(s): Katherine R. Heal, Natalie A. Kellogg, Laura T. Carlson, Regina M. Lionheart, Anitra E. IngallsAbstractDiatoms perform an estimated 20% of global photosynthesis, form the base of the marine food web, and sequester carbon into the deep ocean through the biological pump. In some areas of the ocean, diatom growth is limited by the micronutrient cobalamin (vitamin B12), yet the biochemical ramifications of cobalamin limitation are not well understood. In a laboratory setting, we grew the diatom Thalassiosira pseudonana under replete and low cobalamin condi...
Source: Protist - May 28, 2019 Category: Molecular Biology Source Type: research

From the Popularization of Microscopy in the Victorian Age: A Lesson for Today's “Outreach”
Publication date: Available online 25 May 2019Source: ProtistAuthor(s): John R. DolanIn the latter half of the Victorian Age (1837–1901) microscopy was introduced as popular past-time. Many books were published aimed at general audiences, both adult and juvenile, on microscopy. Here I consider 5 of these popular books of particular interest to protistologists as they included presentations of 'infusoria' or 'animalcules'. I focus on the scientific backgrounds of the authors, from what we know of them, and the approaches taken to engage the reader based on their texts and illustrations. The possible lesson to be drawn...
Source: Protist - May 27, 2019 Category: Molecular Biology Source Type: research

Cosmopolitan Metapopulations?
Publication date: Available online 10 May 2019Source: ProtistAuthor(s): Tom Fenchel, Bland J. Finlay, Genoveva F. Esteban (Source: Protist)
Source: Protist - May 12, 2019 Category: Molecular Biology Source Type: research

Acidocalcisomes: Ultrastructure, Biogenesis, and Distribution in Microbial Eukaryotes
This report describes acidocalcisomes that were snap-frozen in living cells, primarily the green alga Chlamydomonas reinhardtii, and then fractured and etched (QFDEEM). Polyphosphate granules prove to be uncommon in log-phase C. reinhardtii cells and abundant in stressed cells, where they are also found within autophagy-related vacuoles. Their E (ectoplasmic) fracture face adopts a unique rugose morphology with etching, and displays ∼14 nm globular domains in broken cell preparations. Using etched membrane morphology as a guide, acidocalcisomes were identified during assembly in the trans-Golgi and were recognized...
Source: Protist - May 10, 2019 Category: Molecular Biology Source Type: research

Filter-feeding in Colonial Protists
Publication date: Available online 2 May 2019Source: ProtistAuthor(s): Tom FenchelMany protists form cell colonies. Among them several are filter-feeders depending on suspended food particles such as bacteria. It has been suggested that the formation of colonies enhances feeding efficiency and implied that − in the case of colonial choanoflagellates − it was an adaptive trait that led to the evolution of metazoans. Here it is shown experimentally − for a colonial peritrich ciliate and for a choanoflagellate − that colony-formation does not enhance the efficiency of filter-feeding relative to solitar...
Source: Protist - May 4, 2019 Category: Molecular Biology Source Type: research

Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species
Publication date: Available online 13 April 2019Source: ProtistAuthor(s): Galina Prokopchuk, Daria Tashyreva, Akinori Yabuki, Aleš Horák, Petra Masařová, Julius LukešUntil now, Hemistasia phaeocysticola was the only representative of the monogeneric family Hemistasiidae available in culture. Here we describe two new axenized hemistasiids isolated from Tokyo Bay, Japan. Like in other diplonemids, cellular organization of these heterotrophic protists is characterized by a distinct apical papilla, a tubular cytopharynx contiguous with a deep flagellar pocket, and a highly branched mitochondrion wi...
Source: Protist - April 13, 2019 Category: Molecular Biology Source Type: research

Genomics, Biology and Phylogeny Aurantiochytrium acetophilum sp. nov. (Thraustrochytriaceae), Including First Evidence of Sexual Reproduction
We described our new organism as Aurantiochytrium acetophilum sp. nov. (Thraustochytriaceae, Thraustocytriales) using light microscopy, electron microscopy, substrate assimilation, biochemical composition and nuclear genomic data. We found some characteristics of biotechnological relevance that were not previously described in this family. First, strain HS-399 of A. acetophilum was extremely tolerant to acetate toxicity, and it used this substrate as a sole carbon source. Second, we observed putative gametes that fused together to form a zygote. Zygote fate and the life stage with meiosis were not determined; however, we f...
Source: Protist - March 27, 2019 Category: Molecular Biology Source Type: research

Ploeotids Represent Much of the Phylogenetic Diversity of Euglenids
Publication date: Available online 23 March 2019Source: ProtistAuthor(s): Gordon Lax, Won Je Lee, Yana Eglit, Alastair SimpsonPloeotids are an assemblage of rigid phagotrophic euglenids that have 10–12 pellicular strips and glide on their posterior flagellum. Molecular phylogenies place them as a poorly resolved, likely paraphyletic assemblage outside the Spirocuta clade of flexible euglenids, which includes the well-known phototrophs and primary osmotrophs. Here, we report SSU rRNA gene sequences from 38 ploeotids, using both single-cell and culture-based methods. Several contain group I or non-canonical introns. Ou...
Source: Protist - March 24, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: February 2019Source: Protist, Volume 170, Issue 1Author(s): (Source: Protist)
Source: Protist - March 21, 2019 Category: Molecular Biology Source Type: research

Discovery of a New Clade Nested Within the Genus Alexandrium (Dinophyceae): Morpho-molecular Characterization of Centrodinium punctatum (Cleve) F.J.R. Taylor
Publication date: Available online 28 February 2019Source: ProtistAuthor(s): Zhun Li, Kenneth Neil Mertens, Elisabeth Nézan, Nicolas Chomérat, Gwenael Bilien, Mitsunori Iwataki, Hyeon Ho ShinInvestigation of phytoplankton from East China Sea of the Pacific Ocean, offshore Réunion Island of the Indian Ocean, and the French Atlantic coast revealed a species of poorly known armored fusiform dinoflagellate. To clarify this species, morphology and phylogeny based on mitochondrial and nuclear protein gene sequence (Cox1, Cob and Hsp90) concatenated with the SSU, ITS region and LSU rDNA sequences were analyse...
Source: Protist - March 1, 2019 Category: Molecular Biology Source Type: research

Time Calibrated Morpho-molecular Classification of Nassellaria (Radiolaria)
Publication date: Available online 22 February 2019Source: ProtistAuthor(s): Miguel M. Sandin, Loic Pillet, Tristan Biard, Camille Poirier, Estelle Bigeard, Sarah Romac, Noritoshi Suzuki, Fabrice NotNassellaria are marine protists belonging to the Radiolaria lineage (Rhizaria). Their skeleton, made of opaline silica, exhibit an excellent fossil record, extremely valuable in micro-paleontological studies for paleo-environmental reconstruction. Yet, to date very little is known about the extant diversity and ecology of Nassellaria in contemporary oceans, and most of it is inferred from their fossil record. Here we present an...
Source: Protist - February 23, 2019 Category: Molecular Biology Source Type: research

Mapping the Single Origin of Replication in the Naegleria gruberi Extrachromosomal DNA Element
We reported elsewhere the molecular cloning and the complete sequence analysis of the entire rRNA gene-containing element of N. gruberi (strain EGB). Using neutral/neutral two-dimensional agarose electrophoresis, the region in the element enclosing the single replication origin using DNA from asynchronous and axenically propagated N. gruberi populations was localized within a 2.1 kbp fragment located approximately 2,300 bp from the 18S rRNA gene and 3,700 bp from the 28S rRNA gene. The results indicate that replication occurs from a single origin via a theta-type mode of replication rather than by a rolling circl...
Source: Protist - February 14, 2019 Category: Molecular Biology Source Type: research

Survival and growth in vitro of Paramoeba perurans populations cultured under different salinities and temperatures
Publication date: Available online 21 December 2018Source: ProtistAuthor(s): Catherine Collins, Malcolm Hall, Mark J. Fordyce, Patricia WhiteAbstractGrowth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 &...
Source: Protist - December 22, 2018 Category: Molecular Biology Source Type: research

Tuberlatum coatsi gen. n., sp. n. (Alveolata, Perkinsozoa), a New Parasitoid with Short Germ Tubes Infecting Marine Dinoflagellates
Publication date: Available online 21 December 2018Source: ProtistAuthor(s): Boo Seong Jeon, Myung Gil ParkPerkinsozoa is an exclusively parasitic group within the alveolates and infections have been reported from various organisms, including marine shellfish, marine dinoflagellates, freshwater cryptophytes, and tadpoles. Despite its high abundance and great genetic diversity revealed by recent environmental rDNA sequencing studies, Perkinsozoa biodiversity remains poorly understood. During the intensive samplings in Korean coastal waters during June 2017, a new parasitoid of dinoflagellates was detected and was successful...
Source: Protist - December 21, 2018 Category: Molecular Biology Source Type: research

Cryptic Diversity: a Long-lasting Issue for Diatomologists
Publication date: February 2019Source: Protist, Volume 170, Issue 1Author(s): Alberto Amato, Wiebe H.C.F. Kooistra, Marina Montresor (Source: Protist)
Source: Protist - December 14, 2018 Category: Molecular Biology Source Type: research

Isosporan Oocysts in the faeces of bank Voles (Myodes glareolus; Arvicolinae, Rodentia): Real Parasites, or Pseudoparasites?
This study reveals the origin of isosporan oocysts found in faeces of bank voles based on morphological analyses, phylogenetic analyses, and experimental infections. Morphological analyses showed the presence of the Stieda body complex on sporocysts. Phylogenetic analyses demonstrated close phylogenetic relationships between Isospora from bank voles and avian isosporans. Experimental inoculations of bank voles with sporulated oocysts of Isospora did not result in the production of unsporulated oocysts. Hence, these organisms should be considered pseudoparasites of the bank voles/rodents (probably originating from avian Iso...
Source: Protist - December 12, 2018 Category: Molecular Biology Source Type: research

A Novel Phagomyxid Parasite Produces Sporangia in Root Hair Galls of Eelgrass (Zostera marina)
The objective of this study was to identify the parasite causing the formation of root hair galls on eelgrass (Zostera marina) in Puget Sound, WA. Microscopic and molecular analyses revealed that a novel protist formed plasmodia that developed into sporangia in root hair tip galls and released biflagellate swimming zoospores. Root hair galls were also observed in the basal section of root hairs, and contained plasmodia or formed thick-walled structures filled with cells (resting spores). Phylogenetic analyses of 18S rDNA sequence data obtained from cells in sporangia indicated that the closest relative of the parasite with...
Source: Protist - December 11, 2018 Category: Molecular Biology Source Type: research

Erratum to “The Uncoupled Assimilation of Carbon and Nitrogen from Urea and Glycine by the Bloom-forming Dinoflagellate Prorocentrum minimum” [Protist 169 (November (5)) (2018) 603–614]
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): Olga Matantseva, Ilya Pozdnyakov, Maren Voss, Iris Liskow, Sergei Skarlato (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research

Erratum to “Nutritional Intake by Ectoplasmic Nets of Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles” [Protist 169 (November (5)) (2018) 727–743]
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): Izumi Iwata, Daiske Honda (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research

New Selectable Markers for Volvox carteri Transformation
Publication date: Available online 15 November 2018Source: ProtistAuthor(s): José A. Ortega-Escalante, Owen Kwok, Stephen M. MillerVolvox carteri is an excellent model for investigating the evolution of multicellularity and cell differentiation, and the rate of future progress with this system will depend on improved molecular genetic tools. Several selectable markers for nuclear transformation of V. carteri have been developed, including the nitrate reductase (nitA) gene, but it would be useful to have additional markers to multiplex transgenes in this species. To further facilitate molecular genetic analyses of V....
Source: Protist - November 16, 2018 Category: Molecular Biology Source Type: research

Alternative Type II NAD(P)H Dehydrogenases in the Mitochondria of Protists and Fungi
Publication date: Available online 10 November 2018Source: ProtistAuthor(s): Nina Antos-Krzeminska, Wieslawa JarmuszkiewiczPlants, fungi, and some protists possess a more branched electron transport chain in their mitochondria compared to canonical one. In these organisms, the electron transport chain contains several rotenone-insensitive NAD(P)H dehydrogenases. Some are located on the outer surface, and others are located on the inner surface of the inner mitochondrial membrane. The putative role of these enzymes still remains elusive, but they may prevent the overreduction of the electron transport chain components and d...
Source: Protist - November 11, 2018 Category: Molecular Biology Source Type: research

Non-linear physiology and gene expression responses of Harmful Alga Heterosigma akashiwo to Rising CO2
Publication date: Available online 24 October 2018Source: ProtistAuthor(s): Gwenn M.M. Hennon, Olivia M. Williamson, María D. Hernández Limón, Sheean T. Haley, Sonya T. DyhrmanHeterosigma akashiwo is a raphidophyte known for forming ichthyotoxic blooms. In order to predict the potential impacts of rising CO2 on H. akashiwo it is necessary to understand the factors influencing growth rates over a range of CO2 concentrations. Here we examined the physiology and gene expression response of H. akashiwo to concentrations from 200 to 1000 ppm CO2. Growth rate data were combined from this and previous st...
Source: Protist - October 25, 2018 Category: Molecular Biology Source Type: research