Identification and characterization of the cellular subclones that contribute to the pathogenesis of mantle cell lymphoma

Publication date: Available online 21 December 2018Source: Genes & DiseasesAuthor(s): Junling Tang, Li Zhang, Tiejun Zhou, Zhiwei Sun, Liangsheng Kong, Li Jing, Hongyun Xing, Hongyan Wu, Yongli Liu, Shixia Zhou, Jingyuan Li, Mei Chen, Fang Xu, Jirui Tang, Tao Ma, Min Hu, Dan Liu, Jing Guo, Xiaofeng Zhu, Yan ChenAbstractMantle cell lymphoma (MCL) is a B-cell malignancy with poor clinical outcome and undefined pathogenesis. Development of clinically relevant cellular models for MCL research is an urgent need. Our preliminary observations lead the development of two novel hypotheses that we tested in this study: 1. multicellular spheroid might be a unique growth mode of early-stage cells in MCL; 2. MCL might be a polyclonal tumor. We made the following original observations that have not been reported: First, we have provided a new experiment method for enriching MCL early-stage cells and characterized the spheroid mode of growth as a unique feature of early-stage MCL cells in cell line as well as in clinical samples. Second, we have established a clinically relevant cellular model of MCL, the JeKo-1-spheroid cell line, that was highly enriched in early-stage sub-clones. JeKo-1-spheroid cells and the spheroid growing cells enriched from MCL patients exhibited comparably enhanced tumorigenic abilities and similar biological features. Third, Immunophenotypic analysis has revealed that MCL may be derived from precursor-B(pre-B), immature-B and mature-B cells, not only the mature-B ...
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research