A Track of the Clones: New developments in cellular barcoding

Methods to unambiguously mark individual cells and follow their clonal progeny over extended periods of time can provide unprecedented insights into the organizational principles operative in tissue homeostasis and regeneration. The potential of clonal tracking was initially demonstrated by studies of glucose-6-phosphate dehydrogenase (G-6-PD) cellular mosaicism [1,2]. More recently, viral integration site analysis (IS) [3-5] and integration of unique genetic “barcode” sequences [6-9] have been complemented by inducible transposon systems [10,11] and the recombination of multiple segments separated by loxP sites to generate uniquely identifiable barcodes in vivo [12].

Source: Experimental Hematology - November 15, 2018 Category: Hematology Authors: Anne-Marie Lyne, David G Kent, Elisa Laurenti, Kerstin Cornils, Ingmar Glauche, Leila Peri é Source Type: research