Epitope ‐based immunoinformatics approach on RNA‐dependent RNA polymerase (RdRp) protein complex of Nipah virus (NiV)

Persistent outbreaks of Nipah virus (NiV) with severe case fatality throw a major challenge on researchers to develop a drug or vaccine to combat the disease. With little knowledge of its molecular mechanisms, we utilized the proteome data of NiV to evaluate the potency of three major proteins (phosphoprotein, polymerase, and nucleocapsid protein RdRp complex) to count as a possible candidate for epitope ‐based vaccine design. AbstractPersistent outbreaks of Nipah virus (NiV) with severe case fatality throw a major challenge on researchers to develop a drug or vaccine to combat the disease. With little knowledge of its molecular mechanisms, we utilized the proteome data of NiV to evaluate the potency of three major proteins (phosphoprotein, polymerase, and nucleocapsid protein) in the RNA ‐dependent RNA polymerase complex to count as a possible candidate for epitope‐based vaccine design. Profound computational analysis was used on the above proteins individually to explore the T‐cell immune properties like antigenicity, immunogenicity, binding to major histocompatibility comple x class I and class II alleles, conservancy, toxicity, and population coverage. Based on these predictions the peptide ‘ELRSELIGY’ of phosphoprotein and ‘YPLLWSFAM’ of nulceocapsid protein were identified as the best‐predicted T‐cell epitopes and molecular docking with human leukocyte an tigen‐C (HLA‐C*12:03) molecule was effectuated followed by validation with molecular dynamic...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research