Organophosphorus Hydrolase-Poly-β-Cyclodextrin as a Stable Self-Decontaminating Bio-catalytic Material for Sorption and Degradation of Organophosphate Pesticide

Publication date: Available online 2 November 2018Source: Journal of Hazardous MaterialsAuthor(s): Youngkwang Moon, Ali Turab Jafry, Soon Bang Kang, Jin Young Seo, Kyung-Youl Baek, Eui-Joong Kim, Jae-Gu Pan, Jae-Youl Choi, Hyunji Kim, Kang Han Lee, Keunhong Jeong, Seunghan Shin, Jinkee Lee, Yongwoo LeeABSTRACTA region suffering from an attack of a nerve agent requires not only a highly sorptive material but also a fast-acting catalyst to decontaminate the lethal chemical present. The product should be capable of high sorptive capacity, selectivity and quick response time to neutralize the long lasting harmful effects of nerve agents. Herein, we have utilized organophosphorus hydrolase (OPH) as a non-toxic bio-catalytic material held in with the supporting matrix of poly-β-cyclodextrin (PCD) as a novel sorptive reinforced self-decontaminating material against organophosphate intoxication. OPH coated PCD (OPH-PCD) will not only be providing support for holding enzyme but also would be adsorbing methyl paraoxon (MPO) used as a simulant, in a host-guest inclusion complex formation. Sorption trend for PCD revealed preference towards the more hydrophobic MPO against para-nitrophenol (pNP). The results show sorption capacity of 1.26 mg/g of 100 µM MPO with PCD which was 1.7 times higher compared to pNP. The reaction rate with immobilized OPH-PCD was found to be 23 % less compared to free enzyme. With the help of OPH-PCD, continuous hydrolysis (100 %) of MPO into pNP was observed...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research