Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation

In this study a new cryogenic pot, suitable for tomography experiments, has been developed. The pot can rotate a biological cell over an angular range of ± 170 ° against the direction of the incident X-ray beam. Herein, the details and the performance of the pot and miscellaneous devices are reported, along with established experimental procedures including specimen preparation. The apparatus has been used in tomography experiments for visualizing the three-dimensional structure of a Cyanidioschyzon merolae cell with an approximate size of 5   µ m at a resolution of 136   nm. Based on the experimental results, the necessary improvements for future experiments and the resolution limit achievable under experimental conditions within a maximum tolerable dose are discussed.

Source: Journal of Synchrotron Radiation - October 22, 2018 Category: Physics Authors: Kobayashi, A. Takayama, Y. Okajima, K. Oide, M. Yamamoto, T. Sekiguchi, Y. Oroguchi, T. Nakasako, M. Kohmura, Y. Yamamoto, M. Hoshi, T. Torizuka, Y. Tags: X-ray diffraction imaging tomography biological cells cryogenic experiments research papers Source Type: research