Binding of Pneumocystis carinii to the lung epithelial cell receptor HSPA5 (GRP78).

Binding of Pneumocystis carinii to the lung epithelial cell receptor HSPA5 (GRP78).
J Med Microbiol. 2018 Oct 17;:
Authors: Kottom TJ, Hebrink DM, Limper AH
Abstract
The importance of lung macrophages in Pneumocystis-host interaction is well known, but little is known about the initial binding/colonization of the airway epithelium. Our prior studies have documented cell-signalling events that occur following binding of the organisms to lung epithelial cells; however, the receptors that mediate Pneumocystis attachment to lung surfaces have not yet been fully defined. Using affinity chromatography, we identified heat shock protein 5 (HSPA5), also known as GRP78, as a potential host receptor that may have relevance in Pneumocystis lung colonization. Pneumocystis carinii (Pc) organisms not only bound HSPA5 on a rat lung epithelial cell line, but also on primary rat airway epithelial cells (AECs). Furthermore, Pc bound CHO1 cells overexpressing HSPA5 more than the CHO1 parent line alone, supporting a role for Pc-HSPA5 protein interaction in mediating organism attachment. These results provide new insights into the interactions of Pneumocystis with host lung epithelium.
PMID: 30328808 [PubMed - as supplied by publisher]

Source: Journal of Medical Microbiology - October 17, 2018 Category: Microbiology Authors: Kottom TJ, Hebrink DM, Limper AH Tags: J Med Microbiol Source Type: research