Improved conversion of Dibenzothiophene into sulfone by surface display of Dibenzothiophene monooxygenase (DszC) in recombinant Escherichia coli

Publication date: Available online 12 October 2018Source: Journal of BiotechnologyAuthor(s): Sabita Rangra, Mira Kabra, Vageesh Gupta, Preeti SrivastavaAbstractBiodesulfurization is an eco-friendly process for removing sulfur from petroleum fractions. The process could not be commercialized because of the inability of microorganisms to desulfurize a wide range of heterocyclic poly aromatic sulfur compounds like dibenzothiophene (DBT), 4, 6-Dimethyl DBT present in fuel and low desulfurization activity. In the present study, to improve the rates of conversion of dibenzothiophene to dibenzothiophene sulfone, the responsible enzyme dibenzothiophene monooxygenase DszC, is displayed on the surface of Escherichia coli. This helped in overcoming the mass transfer limitation and resulted in approximately 3 times faster conversion with respect to control (which contained intracellular enzyme). This is the first report demonstrating display of a biodesulfurization enzyme on bacterial cell surface.
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research