Cancers, Vol. 10, Pages 343: Characterization of Precursor-Dependent Steroidogenesis in Human Prostate Cancer Models

Cancers, Vol. 10, Pages 343: Characterization of Precursor-Dependent Steroidogenesis in Human Prostate Cancer Models Cancers doi: 10.3390/cancers10100343 Authors: Subrata Deb Steven Pham Dong-Sheng Ming Mei Yieng Chin Hans Adomat Antonio Hurtado-Coll Martin E. Gleave Emma S. Tomlinson Guns Castration-resistant prostate tumors acquire the independent capacity to generate androgens by upregulating steroidogenic enzymes or using steroid precursors produced by the adrenal glands for continued growth and sustainability. The formation of steroids was measured by liquid chromatography-mass spectrometry in LNCaP and 22Rv1 prostate cancer cells, and in human prostate tissues, following incubation with steroid precursors (22-OH-cholesterol, pregnenolone, 17-OH-pregnenolone, progesterone, 17-OH-progesterone). Pregnenolone, progesterone, 17-OH-pregnenolone, and 17-OH-progesterone increased C21 steroid (5-pregnan-3,20-dione, 5-pregnan-3,17-diol-20-one, 5-pregnan-3-ol-20-one) formation in the backdoor pathway, and demonstrated a trend of stimulating dihydroepiandrosterone or its precursors in the backdoor pathway in LNCaP and 22Rv1 cells. The precursors differentially affected steroidogenic enzyme messenger RNA (mRNA) expressions in the cell lines. The steroidogenesis following incubation of human prostate tissue with 17-OH-pregnenolone and progesterone produced trends similar to those observed in cell lines. Interestingly, the formation of C21 steroids from classical...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research