Ultra-performance liquid chromatography tandem mass-spectrometry (UHPLC-MS/MS) for the rapid, simultaneous analysis of ziprasidone and its impurities.

Ultra-performance liquid chromatography tandem mass-spectrometry (UHPLC-MS/MS) for the rapid, simultaneous analysis of ziprasidone and its impurities. Biomed Chromatogr. 2018 Sep 14;:e4384 Authors: Čarapić M, Nikolic K, Marković B, Petković M, Pavlovic M, Agbaba D Abstract Separation and characterization of the unknown degradation product of second generation antipsychotic drug ziprasidone was essential for defining genotoxic potential of the compound. The aim of this study was to develop simple UHPLC method coupled with tandem mass spectrometry (MS/MS) for chemical characterization of unknown degradant, separation and quantification of ziprasidone and its five main impurities (I-V) in raw material and pharmaceuticals. Chromatographic conditions were optimized by experimental design. The MS/MS fragmentation conditions were optimized individually for each compound in order to obtain both specific fragments and high signal intensity. A rapid and sensitive UHPLC-MS/MS method was developed. All seven analytes were eluted within the 7 min run time. The best separation was obtained on the Acquity UPLC BEH C18 (50 mm x 2.1 mm x 1.7 μm) column in gradient mode with ammonium-formate buffer (10mM; pH 4.7) and acetonitrile as mobile phase, with the flow rate of 0.3 mL min-1 and at the column temperature of 30o C. The new UHPLC-MS/MS method was fully validated and all validation parameters were confirmed. The fragmentation pathways and chem...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research